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EDC4 Polyclonal antibody

EDC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17737-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Autoantigen Ge-1, Autoantigen RCD-8, Enhancer of mRNA-decapping protein 4, Ge 1, Hedls

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EDC4 Ready-To-Use IHC Kit. EDC4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17737-1-AP targets EDC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 EDC4 fusion protein Ag11784 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enhancer of mRNA decapping 4
别名 Autoantigen Ge-1, Autoantigen RCD-8, Enhancer of mRNA-decapping protein 4, Ge 1, Hedls
计算分子量 1401 aa, 152 kDa
观测分子量 160 kDa
GenBank蛋白编号BC064567
基因名称 EDC4
Gene ID (NCBI) 23644
RRIDAB_10665813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P2E9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enhancer of mRNA-decapping protein 4 (EDC4) is a key regulator of mRNA decapping and degradation, playing a crucial role in the mRNA decay pathway. It is involved in the formation of processing bodies (P-bodies) in the cytoplasm, where it interacts with other decapping factors such as DCP1A and DCP2A to facilitate mRNA degradation (PMID: 16364915). EDC4 also interacts with the mammalian target of rapamycin complex 1 (mTORC1) and is involved in the regulation of immune system functions (PMID: 25514416; 25970328). Additionally, EDC4 has been shown to regulate DNA repair processes, with its deficiency leading to genome instability and hypersensitivity to DNA interstrand cross-linking drugs (PMID: 29511213).

实验方案

Product Specific Protocols
WB protocol for EDC4 antibody 17737-1-APDownload protocol
IHC protocol for EDC4 antibody 17737-1-APDownload protocol
IF protocol for EDC4 antibody 17737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
humanWB

Cancer Res

Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition

Authors - Liang Fang
humanIF

Acta Pharmacol Sin

NSCLC cells sustain phase separation of cytoplasmic membrane-less organelles to protect themselves against cisplatin treatment

Authors - Ning-Ning Li
humanWB

Mol Ther Nucleic Acids

The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.

Authors - Tao Liu
mouseWB

J Genet Genomics

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

Authors - Huiru Zhang
humanIF

FEBS Lett

Insight into the function of the Golgi membrane protein GOLM1 in cholangiocytes through interactomic analysis

Authors - Meghana Nagaraj
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