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验证数据展示

  • WB analysis of A549 using 18032-1-AP (same clone as 18032-1-PBS)

    WB analysis of A549 using 18032-1-AP (same clone as 18032-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18032-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 18032-1-AP (same clone as 18032-1-PBS)

    IP experiment of A549 using 18032-1-AP (same clone as 18032-1-PBS)

    IP result of anti-EDAR (IP:18032-1-AP, 4ug; Detection:18032-1-AP 1:500) with A549 cells lysate 2800ug. This data was developed using the same antibody clone with 18032-1-PBS in a different storage buffer formulation.

  • WB analysis using 18032-1-AP (same clone as 18032-1-PBS)

    WB analysis using 18032-1-AP (same clone as 18032-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18032-1-PBS in a different storage buffer formulation.

EDAR Polyclonal antibody, PBS Only

EDAR Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 18032-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18032-1-PBS targets EDAR in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12601

Product name: Recombinant human EDAR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 207-448 aa of BC093872

Sequence: MFYILKTKPSAPACCTSHPGKSVEAQVSKDEEKKEAPDNVVMFSEKDEFEKLTATPAKPTKSENDASSENEQLLSRSVDSDEEPAPDKQGSPELCLLSLVHLAREKSATSNKSAGIQSRRKKILDVYANVCGVVEGLSPTELPFDCLEKTSRMLSSTYNSEKAVVKTWRHLAESFGLKRDEIGGMTDGMQLFDRISTAGYSIPELLTKLVQIERLDAVESLCADILEWAGVVPPASQPHAAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ectodysplasin A receptor
别名
计算分子量 448 aa, 49 kDa
观测分子量 48 kDa
GenBank蛋白编号BC093872
基因名称 EDAR
Gene ID (NCBI) 10913
RRIDAB_10596932
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNE0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.

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