验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, A431 cells, MCF-7 cells |
| Positive IP detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18032-1-AP targets EDAR in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag12601 Product name: Recombinant human EDAR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 207-448 aa of BC093872 Sequence: MFYILKTKPSAPACCTSHPGKSVEAQVSKDEEKKEAPDNVVMFSEKDEFEKLTATPAKPTKSENDASSENEQLLSRSVDSDEEPAPDKQGSPELCLLSLVHLAREKSATSNKSAGIQSRRKKILDVYANVCGVVEGLSPTELPFDCLEKTSRMLSSTYNSEKAVVKTWRHLAESFGLKRDEIGGMTDGMQLFDRISTAGYSIPELLTKLVQIERLDAVESLCADILEWAGVVPPASQPHAAS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ectodysplasin A receptor |
| 别名 | DL, Downless homolog, Ectodermal dysplasia receptor, ectodysplasin A receptor, Ectodysplasin-A receptor |
| 计算分子量 | 448 aa, 49 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC093872 |
| 基因名称 | EDAR |
| Gene ID (NCBI) | 10913 |
| RRID | AB_10596932 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNE0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for EDAR antibody 18032-1-AP | Download protocol |
| IP protocol for EDAR antibody 18032-1-AP | Download protocol |
| WB protocol for EDAR antibody 18032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Development Involvement of Wnt, Eda and Shh at defined stages of sweat gland development. | ||
J Pharm Pharmacol Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway. | ||
J Histochem Cytochem Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice | ||
J Invest Dermatol ASH2L mediates epidermal differentiation and hair follicle morphogenesis via H3K4me3 modification |
