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验证数据展示

  • WB analysis of MCF-7 using 66023-1-Ig

    WB analysis of MCF-7 using 66023-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66023-1-Ig

    IHC staining of human breast cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ECM1 antibody included
  • IHC staining of human breast cancer using 66023-1-Ig

    IHC staining of human breast cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ECM1 antibody included

ECM1 Monoclonal antibody

ECM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66023-1-Ig

产品说明书

CloneNo. 6A12B3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, ELISA

别名

ECM1, extracellular matrix protein 1, Secretory component p85

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, COLO 320 cells, human heart tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66023-1-Ig targets ECM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ECM1 fusion protein Ag17955 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 extracellular matrix protein 1
别名 ECM1, extracellular matrix protein 1, Secretory component p85
计算分子量 540 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC023505
基因名称 ECM1
Gene ID (NCBI) 1893
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16610
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.