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ECHS1 Recombinant antibody

ECHS1 Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5G5

Cat No : 81611-1-RR

Print datasheet

Synonyms

ECHS1, Enoyl CoA hydratase 1, SCEH

验证数据展示

  • WB analysis using 81611-1-RR

    WB analysis using 81611-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 81611-1-RR

    WB analysis using 81611-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 81611-1-RR

    IHC staining of human prostate cancer using 81611-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IF Staining of HeLa using 81611-1-RR

    IF Staining of HeLa using 81611-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ECHS1 antibody (81611-1-RR, Clone: 5G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, L02 cells, Caco-2 cells, MCF-7 cells, HepG2 cells, HEK-293 cells, PC-3 cells, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81611-1-RR targets ECHS1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen ECHS1 fusion protein Ag1842 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Synonyms ECHS1, Enoyl CoA hydratase 1, SCEH
Calculated Molecular Weight 31 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC008906
Gene Symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

实验方案

Product Specific Protocols
WB protocol for ECHS1 antibody 81611-1-RRDownload protocol
IHC protocol for ECHS1 antibody 81611-1-RRDownload protocol
IF protocol for ECHS1 antibody 81611-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols