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验证数据展示

  • WB analysis using 81611-1-RR

    WB analysis using 81611-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 81611-1-RR

    WB analysis using 81611-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 81611-1-RR

    WB analysis of MCF-7 using 81611-1-RR

    WB result of ECHS1 antibody (81611-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells.

  • IHC staining of human prostate cancer using 81611-1-RR

    IHC staining of human prostate cancer using 81611-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IF Staining of HeLa using 81611-1-RR

    IF Staining of HeLa using 81611-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ECHS1 antibody (81611-1-RR, Clone: 5G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 81611-1-RR

    Affinity and Kinetic Characterization of 81611-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81611-1-RR against Human ECHS1 were performed. The affinity constant is 95.5 pM.

  • Featured Product
  • KD/KO验证

ECHS1 Recombinant monoclonal antibody

ECHS1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 81611-1-RR

产品说明书

CloneNo. 5G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse liver tissue, L02 cells, Caco-2 cells, HepG2 cells, HEK-293 cells, PC-3 cells, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81611-1-RR targets ECHS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1842

Product name: Recombinant human ECHS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC008906

Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
别名 mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17
计算分子量 31 kDa
观测分子量30 kDa
GenBank蛋白编号BC008906
基因名称 ECHS1
Gene ID (NCBI) 1892
RRIDAB_2935566
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30084
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

实验方案

Product Specific Protocols
IF protocol for ECHS1 antibody 81611-1-RRDownload protocol
IHC protocol for ECHS1 antibody 81611-1-RRDownload protocol
WB protocol for ECHS1 antibody 81611-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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