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验证数据展示

  • WB analysis of mouse liver using 11385-1-AP (same clone as 11385-1-PBS)

    WB analysis of mouse liver using 11385-1-AP (same clone as 11385-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 11385-1-AP (same clone as 11385-1-PBS)

    IHC staining of human colon using 11385-1-AP (same clone as 11385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11385-1-AP (ECH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECH1 antibody included

ECH1 Polyclonal antibody, PBS Only

ECH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11385-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

HPXEL, EC:5.3.3.-, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ECH1 Ready-To-Use IHC Kit. ECH1 primary antibody included.

产品信息

11385-1-PBS targets ECH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1931

Product name: Recombinant human ECH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-328 aa of BC017408

Sequence: MAAGIVASRRLRDLLTRRLTGSNYPGLSISLRLTGSSAQEAASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enoyl Coenzyme A hydratase 1, peroxisomal
别名 HPXEL, EC:5.3.3.-, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
计算分子量 328 aa, 36 kDa
观测分子量 30-36 kDa
GenBank蛋白编号BC017408
基因名称 ECH1
Gene ID (NCBI) 1891
RRIDAB_2277810
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13011
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Enoyl coenzyme A hydratase 1 (ECH1), is first recog-nized as a key component in mitochondrial fatty acid β-oxidation, and subsequent studies have demonstrated that it regulates multiple pathophysiological processes (PMID:33813878). The MW of ECH1 is 36 kDa,and can be detected as 30-36 kDa.

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