ECH1 Polyclonal antibody

ECH1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11385-1-AP

Print datasheet

Synonyms

ECH1, HPXEL



经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells, L02 cells, mouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ECH1 Ready-To-Use IHC Kit. ECH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

11385-1-AP targets ECH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ECH1 fusion protein Ag1931 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Synonyms ECH1, HPXEL
Calculated Molecular Weight 328 aa, 36 kDa
Observed Molecular Weight 30-36 kDa
GenBank Accession NumberBC017408
Gene Symbol ECH1
Gene ID (NCBI) 1891
RRIDAB_2277810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ECH1 antibody 11385-1-APDownload protocol
IHC protocol for ECH1 antibody 11385-1-APDownload protocol
IF protocol for ECH1 antibody 11385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang
mouseWB

J Clin Invest

Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.

Authors - Miguel A Lanaspa
mouseWB

Hepatology

High fat and high sucrose (western) diet induce steatohepatitis that is dependent on fructokinase.

Authors - Ishimoto Takuji T
mouseWB

Front Cell Dev Biol

Single-Cell Transcriptomics Reveals Splicing Features of Adult Neural Stem Cells in the Subventricular Zone.

Authors - Yanlu Wang
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi