验证数据展示
经过测试的应用
| Positive WB detected in | human spleen tissue, rat spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66354-1-Ig targets EBI3/IL-27B in WB, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat, pig |
| 免疫原 |
CatNo: Ag3051 Product name: Recombinant human EBI3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC015364 Sequence: MTPQLLLALVLWASCPPCSGRKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Epstein-Barr virus induced 3 |
| 别名 | EBI3, 1F3H10, EBV induced gene 3 protein, EBV-induced gene 3 protein, Epstein Barr virus induced 3 |
| 计算分子量 | 229 aa, 25 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC015364 |
| 基因名称 | EBI3 |
| Gene ID (NCBI) | 10148 |
| RRID | AB_2881734 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14213 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EBI3/IL-27B antibody 66354-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
