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验证数据展示

  • WB analysis of HepG2 using 26465-1-AP

    WB analysis of HepG2 using 26465-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26465-1-AP (EAF6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26465-1-AP

    IP experiment of HepG2 using 26465-1-AP

    IP result of anti-EAF6 (IP:26465-1-AP, 4ug; Detection:26465-1-AP 1:500) with HepG2 cells lysate 3480ug.

EAF6 Polyclonal antibody

EAF6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 26465-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, ELISA

别名

C1orf149, CDABP0189, CENP-28, Chromatin modification-related protein MEAF6, Esa1-associated factor 6 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

26465-1-AP targets EAF6 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag23971

Product name: Recombinant human C1orf149 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-191 aa of BC016328

Sequence: MAMHNKAAPPQIPDTRRELAELVKRKQELAETLANLERQIYAFEGSYLEDTQMYGNIIRGWDRYLTNQKNSNSKNDRRNRKFKEAERLFSKSSVTSAAAVSALAGVQDQLIEKREPGSGTESDTSPDFHNQENEPSQEDPEDLDGSVQGVKPQKAASSTSSGSHHSSHKKRKNKNRHRIDLKLNKKPRADY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 149
别名 C1orf149, CDABP0189, CENP-28, Chromatin modification-related protein MEAF6, Esa1-associated factor 6 homolog
观测分子量 22-28 kDa
GenBank蛋白编号BC016328
基因名称 MEAF6
Gene ID (NCBI) 64769
RRIDAB_2880524
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EAF6 antibody 26465-1-APDownload protocol
IP protocol for EAF6 antibody 26465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Discov

MOZ and Menin-MLL Complexes are Complementary Regulators of Chromatin Association and Transcriptional Output in Gastrointestinal Stromal Tumor.

Authors - Matthew L Hemming
View Article
mouseWB

Cell Death Differ

MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.

Authors - Lifang Hu
View Article