验证数据展示
产品信息
88675-1-PBS targets EAAT2 as part of a matched antibody pair:
MP03768-1: 88675-2-PBS capture and 88675-1-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Sandwich ELISA, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag19816 Product name: Recombinant human SLC1A2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 460-574 aa of BC132768 Sequence: PTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 1 (glial high affinity glutamate transporter), member 2 |
| 别名 | GLT1, SLC1A2, GLT 1, Sodium-dependent glutamate/aspartate transporter 2 |
| 计算分子量 | 574 aa, 62 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC132768 |
| 基因名称 | EAAT2 |
| Gene ID (NCBI) | 6506 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43004 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
There are five isoforms of the EAATs, with their expression being cell type and brain region specific . The isoforms EAAT1 and EAAT2 are primarily expressed on astrocytes, with high expression in the cerebellum and throughout the entire brain, respectively. EAAT3 is expressed throughout the brain, while EAAT4 expression is predominantly found in the cerebellum. EAAT5 expression is mainly restricted to photoreceptors and bipolar cells in the retina. EAAT2, encoded by the SLC1A2 gene and also referred to by its rodent nomenclature, glutamate transporter 1 (GLT-1), is responsible for 90% of glutamate uptake in the mature brain. Its expression is hence critical for controlling extracellular glutamate concentrations.
