验证数据展示
经过测试的应用
| Positive WB detected in | mouse hippocampus tissue, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88675-1-RR targets EAAT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag19816 Product name: Recombinant human SLC1A2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 460-574 aa of BC132768 Sequence: PTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 1 (glial high affinity glutamate transporter), member 2 |
| 别名 | GLT1, SLC1A2, GLT 1, Sodium-dependent glutamate/aspartate transporter 2 |
| 计算分子量 | 574 aa, 62 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC132768 |
| 基因名称 | EAAT2 |
| Gene ID (NCBI) | 6506 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
There are five isoforms of the EAATs, with their expression being cell type and brain region specific . The isoforms EAAT1 and EAAT2 are primarily expressed on astrocytes, with high expression in the cerebellum and throughout the entire brain, respectively. EAAT3 is expressed throughout the brain, while EAAT4 expression is predominantly found in the cerebellum. EAAT5 expression is mainly restricted to photoreceptors and bipolar cells in the retina. EAAT2, encoded by the SLC1A2 gene and also referred to by its rodent nomenclature, glutamate transporter 1 (GLT-1), is responsible for 90% of glutamate uptake in the mature brain. Its expression is hence critical for controlling extracellular glutamate concentrations.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EAAT2 antibody 88675-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
