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E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for WB, IP, IF/ICC,IF-P, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IF/ICC,IF-P, FC, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20874-1-AP

Print datasheet

Synonyms

Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin



经过测试的应用

Positive WB detected inA431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells
Positive IP detected inA431 cells
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human colon tissue, mouse skin tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse colon tissue, A431 cells, mouse small intestine tissue, MCF-7 cells, mouse embryo tissue, human breast cancer tissue
Positive FC detected inHepG2 cells

Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20874-1-AP targets E-cadherin in WB, IP, IF, FC, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC,IF-P, FC, IHC, ELISA
Cited ApplicationsWB, IP, IF, FC, IHC, CoIP, Cell treatment
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, pig, canine, bovine
Immunogen E-cadherin fusion protein Ag14973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Synonyms Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin
Calculated Molecular Weight 882 aa, 97 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC141838
Gene Symbol CDH1
Gene ID (NCBI) 999
RRIDAB_10697811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12830
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

实验方案

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-APDownload protocol
IHC protocol for E-cadherin antibody 20874-1-APDownload protocol
IF protocol for E-cadherin antibody 20874-1-APDownload protocol
IP protocol for E-cadherin antibody 20874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.

Authors - Yanpu Chen
human,mouseWB,IHC

Mol Cancer

lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2

Authors - Jin Wang
human, rat, mouseWB

Sci Transl Med

MG53-mediated cell membrane repair protects against acute kidney injury.

Authors - Pu Duann
mouseWB

ACS Nano

Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.

Authors - Han Lin
human,mouseWB,IHC,IF,FC

Nat Commun

TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity

Authors - Sujin Kang
humanWB

Nat Commun

UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis

Authors - Jeonghyun Kim