• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84646-3-RR (same clone as 84646-3-PBS)

    WB analysis using 84646-3-RR (same clone as 84646-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84646-3-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 84646-3-RR (same clone as 84646-3-PBS)

    IHC staining of rat colon using 84646-3-RR (same clone as 84646-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 84646-3-RR (same clone as 84646-3-PBS)

    IHC staining of rat colon using 84646-3-RR (same clone as 84646-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01483-1

    Sandwich ELISA standard curve of MP01483-1

    Sandwich ELISA standard curve of MP01483-1, Rat E-Cadherin Recombinant Matched Antibody Pair - PBS only. 84646-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1229. 84646-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01483-1

    Sample test of MP01483-1

    Urine of six rats was measured. The E-Cadherin concentration of detected samples was determined to be 5,367.9 ng/mL with a range of 1,627.0-10,091.3 ng/mL.

  • Sample test of MP01483-1

    Sample test of MP01483-1

    The mean E-Cadherin concentration was determined to be 33.3 ng/mL in rat spleen tissue extract based on a 2.7 mg/mL extract load.

  • Sample test of MP01483-1

    Sample test of MP01483-1

    Serum of six rats was measured. The E-Cadherin concentration of detected samples was determined to be 673.3 ng/mL with a range of 608.2-748.3 ng/mL.

  • Cytometric bead array standard curve of MP01483-1

    Cytometric bead array standard curve of MP01483-1

    Cytometric bead array standard curve of MP01483-1, RAT E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84646-1-PBS. Detection antibody: 84646-3-PBS. Standard: Eg1229. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01483-2

    Cytometric bead array standard curve of MP01483-2

    Cytometric bead array standard curve of MP01483-2, RAT E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84646-2-PBS. Detection antibody: 84646-3-PBS. Standard: Eg1229. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84646-3-RR

    Affinity and Kinetic Characterization of 84646-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84646-3-RR against Rat E-Cadherin were performed. The affinity constant is 1.84 nM.

Rat E-Cadherin Recombinant antibody, PBS Only (Detector)

E-Cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84646-3-PBS

产品说明书

CloneNo. 242018D3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

242018D3, Cadherin-1, CD324, Cdh1, E-Cad/CTF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84646-3-PBS targets E-Cadherin as part of a matched antibody pair:

MP01483-1: 84646-1-PBS capture and 84646-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01483-2: 84646-2-PBS capture and 84646-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, rat
免疫原 E-Cadherin fusion protein Eg1229 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1
别名 242018D3, Cadherin-1, CD324, Cdh1, E-Cad/CTF1
计算分子量99 kDa
观测分子量125 kDa
GenBank蛋白编号NM_031334.1
基因名称 Cdh1
Gene ID (NCBI) 83502
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9R0T4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.