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验证数据展示

  • WB analysis using 84111-2-RR (same clone as 84111-2-PBS)

    WB analysis using 84111-2-RR (same clone as 84111-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84111-2-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84111-2-RR (same clone as 84111-2-PBS)

    IHC staining of mouse colon using 84111-2-RR (same clone as 84111-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-2-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84111-2-RR (same clone as 84111-2-PBS)

    IHC staining of mouse colon using 84111-2-RR (same clone as 84111-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-2-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84111-2-RR (same clone as 84111-2-PBS)

    IF Staining of A431 using 84111-2-RR (same clone as 84111-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-Cadherin antibody (84111-2-RR, Clone: 241282A8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01047-2

    Sandwich ELISA standard curve of MP01047-2

    Sandwich ELISA standard curve of MP01047-2, Mouse E-Cadherin Recombinant Matched Antibody Pair - PBS only. 84111-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0975. 84111-2-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

  • Sample test of MP01047-2

    Sample test of MP01047-2

    The mean E-Cadherin concentration was determined to be 10.6 ng/mL in mouse liver tissue extract based on a 5.0 mg/mL extract load.

  • Sample test of MP01047-2

    Sample test of MP01047-2

    Serum of six mice was measured. The E-Cadherin concentration of detected samples was determined to be 189.4 ng/mL with a range of 166.7-209.1 ng/mL.

  • Cytometric bead array standard curve of MP01047-1

    Cytometric bead array standard curve of MP01047-1

    Cytometric bead array standard curve of MP01047-1, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-4-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01047-2

    Cytometric bead array standard curve of MP01047-2

    Cytometric bead array standard curve of MP01047-2, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-1-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01047-3

    Cytometric bead array standard curve of MP01047-3

    Cytometric bead array standard curve of MP01047-3, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-3-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84111-2-RR

    Affinity and Kinetic Characterization of 84111-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84111-2-RR against Mouse E-Cadherin were performed. The affinity constant is below 1 pM.

Mouse E-Cadherin Recombinant antibody, PBS Only (Detector)

E-Cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84111-2-PBS

产品说明书

CloneNo. 241282A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

241282A8, cadherin 1, Cdh1, Ecad, L CAM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84111-2-PBS targets E-Cadherin as part of a matched antibody pair:

MP01047-1: 84111-4-PBS capture and 84111-2-PBS detection (validated in Cytometric bead array)

MP01047-2: 84111-1-PBS capture and 84111-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01047-3: 84111-3-PBS capture and 84111-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 E-Cadherin fusion protein Eg0975 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1
别名 241282A8, cadherin 1, Cdh1, Ecad, L CAM
计算分子量98kDa
观测分子量135 kDa
GenBank蛋白编号NM_009864.3
基因名称 E-cadherin
Gene ID (NCBI) 12550
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09803
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.