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验证数据展示

  • IHC staining of mouse colon using 84111-1-RR (same clone as 84111-1-PBS)

    IHC staining of mouse colon using 84111-1-RR (same clone as 84111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84111-1-RR (same clone as 84111-1-PBS)

    IHC staining of mouse colon using 84111-1-RR (same clone as 84111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-1-RR (E-Cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01047-2

    Sandwich ELISA standard curve of MP01047-2

    Sandwich ELISA standard curve of MP01047-2, Mouse E-Cadherin Recombinant Matched Antibody Pair - PBS only. 84111-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0975. 84111-2-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

  • Sample test of MP01047-2

    Sample test of MP01047-2

    The mean E-Cadherin concentration was determined to be 10.6 ng/mL in mouse liver tissue extract based on a 5.0 mg/mL extract load.

  • Sample test of MP01047-2

    Sample test of MP01047-2

    Serum of six mice was measured. The E-Cadherin concentration of detected samples was determined to be 189.4 ng/mL with a range of 166.7-209.1 ng/mL.

  • Cytometric bead array standard curve of MP01047-2

    Cytometric bead array standard curve of MP01047-2

    Cytometric bead array standard curve of MP01047-2, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-1-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

Mouse E-cadherin Recombinant antibody, PBS Only (Capture)

E-cadherin Uni-rAbTM Recombinant Antibody for IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84111-1-PBS

产品说明书

CloneNo. 241282A5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

cadherin 1, Cdh1, Ecad, L CAM, Um

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84111-1-PBS targets E-cadherin as part of a matched antibody pair:

MP01047-2: 84111-1-PBS capture and 84111-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 E-cadherin fusion protein Eg0975 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1
别名 cadherin 1, Cdh1, Ecad, L CAM, Um
计算分子量98kDa
GenBank蛋白编号NM_009864.3
基因名称 E-cadherin
Gene ID (NCBI) 12550
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09803
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.