×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 11017-1-AP (same clone as 11017-1-PBS)

    WB analysis using 11017-1-AP (same clone as 11017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

    IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAG1 antibody included
  • IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

    IHC staining of mouse heart using 11017-1-AP (same clone as 11017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAG1 antibody included
  • IP experiment of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

    IP experiment of mouse brain using 11017-1-AP (same clone as 11017-1-PBS)

    IP result of anti-Dystroglycan (IP:11017-1-AP, 4ug; Detection:11017-1-AP 1:500) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

    IF Staining of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

    Immunofluorescent analysis of HeLa cells using 11017-1-AP (Dystroglycan antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

    FC experiment of HeLa using 11017-1-AP (same clone as 11017-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Dystroglycan (11017-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11017-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Dystroglycan Polyclonal antibody, PBS Only

Dystroglycan Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11017-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

DAG1, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy DAG1 Ready-To-Use IHC Kit. DAG1 primary antibody included.

产品信息

11017-1-PBS targets Dystroglycan in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1456

Product name: Recombinant human DAG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 546-895 aa of BC012740

Sequence: VGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDRAPARFKAKFVGDPALVLNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQIAGLSRRIAEDDGKPRPAFSNALEPDFKATSITVTGSGSCRHLQFIPVVPPRRVPSEAPPTEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTMGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dystroglycan 1 (dystrophin-associated glycoprotein 1)
别名 DAG1, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG
计算分子量 97 kDa
观测分子量 43 kDa
GenBank蛋白编号BC012740
基因名称 Dystroglycan
Gene ID (NCBI) 1605
RRIDAB_2090032
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14118
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

||
New chat

Able™

正在加载，请稍候...