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验证数据展示

  • IF Staining of SH-SY5Y using CL647-67589

    IF Staining of SH-SY5Y using CL647-67589

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 647 Drebrin antibody (CL647-67589, Clone: 1A12H1 ) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL647-67589

    FC experiment of SH-SY5Y using CL647-67589

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Drebrin (CL647-67589, Clone:1A12H1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated Drebrin Monoclonal antibody

Drebrin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-67589

产品说明书

CloneNo. 1A12H1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67589-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67589 targets Drebrin in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 Drebrin fusion protein Ag22635 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 drebrin 1
别名 DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1
观测分子量 110-120 kDa
GenBank蛋白编号BC007567
基因名称 Drebrin
Gene ID (NCBI) 1627
ENSEMBL Gene IDENSG00000113758
RRIDAB_3084824
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16643
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Drebrin antibody CL647-67589Download protocol
Standard Protocols
Click here to view our Standard Protocols