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验证数据展示

  • Dot Blot experiment of / using 80240-2-RR

    Dot Blot experiment of / using 80240-2-RR

    Dot blot analysis was used to confirm the specificity of 80240-2-RR Di/Tri-Methyl-Histone H3 (Lys9) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 80240-2-RR

    ChIP experiment of HeLa using 80240-2-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di/Tri-Methyl-Histone H3 (Lys9) (80240-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis of NIH/3T3 using 80240-2-RR

    WB analysis of NIH/3T3 using 80240-2-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80240-2-RR (HIST1H3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80240-2-RR

    WB analysis using 80240-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80240-2-RR (Di/Tri-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 80240-2-RR

    IF Staining of NIH/3T3 using 80240-2-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di/Tri-Methyl-Histone H3 (Lys9) antibody (80240-2-RR, Clone: 241994C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Di/Tri-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody

Di/Tri-Methyl-Histone H3 (Lys9) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
Cat No. 80240-2-RR

产品说明书

CloneNo. 241994C6
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR and More (1)

别名

Di/Tri Methyl Histone H3, Di/Tri-Methyl Histone H3 (Lys9), Di/Tri-Methyl Histone H3 Lys9, Di/Tri-Methyl Histone-H3 Lys9, Di/Tri-Methyl-Histone H3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells
Positive IF/ICC detected inNIH/3T3 cells
Positive Dot Blot detected in/ /
Positive ChIP-qPCR detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
DOT BLOTDOT BLOT : 1:500-1:2000
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

ChIPSee 1 publications below

产品信息

80240-2-RR targets Di/Tri-Methyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR Application Description
文献引用应用ChIP
经测试反应性 human, mouse
文献引用反应性human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Di/Tri Methyl Histone H3, Di/Tri-Methyl Histone H3 (Lys9), Di/Tri-Methyl Histone H3 Lys9, Di/Tri-Methyl Histone-H3 Lys9, Di/Tri-Methyl-Histone H3
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3743336
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.

实验方案

Product Specific Protocols
IF protocol for Di/Tri-Methyl-Histone H3 (Lys9) antibody 80240-2-RRDownload protocol
WB protocol for Di/Tri-Methyl-Histone H3 (Lys9) antibody 80240-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP

Genome Biol

A LINE-1 insertion upstream of FOXP2 promotes neuronal differentiation during primate evolution.

Authors - Jinhao Liu
View Article
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