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验证数据展示

  • Dot Blot experiment of peptide using 82859-1-RR

    Dot Blot experiment of peptide using 82859-1-RR

    Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • Dot Blot experiment of peptide using 82859-1-RR

    Dot Blot experiment of peptide using 82859-1-RR

    Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • WB analysis using 82859-1-RR

    WB analysis using 82859-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 82859-1-RR

    WB analysis of mouse liver using 82859-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Di-Methyl-Histone H4 (Arg3) Recombinant antibody

Di-Methyl-Histone H4 (Arg3) Uni-rAbTM Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 82859-1-RR

产品说明书

CloneNo. 1E21

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Dot Blot, ELISA

别名

Di-Methyl-Histone H4, Di-Methyl Histone-H4 Arg3, Di-Methyl Histone H4 Arg3, Di-Methyl Histone H4 (Arg3), Di Methyl Histone H4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
Positive Dot Blot detected inpeptide

This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H4a
别名 Di-Methyl-Histone H4, Di-Methyl Histone-H4 Arg3, Di-Methyl Histone H4 Arg3, Di-Methyl Histone H4 (Arg3), Di Methyl Histone H4
观测分子量13 kDa
GenBank蛋白编号BC069654
基因名称 HIST1H4A
Gene ID (NCBI) 8359
RRIDAB_3670583
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62805
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Di-Methyl-Histone H4 (Arg3) antibody 82859-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols