• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • Dot Blot experiment of / using 86990-2-RR

    Dot Blot experiment of / using 86990-2-RR

    Dot blot analysis was used to confirm the specificity of 86990-2-RR Di-Methyl-Histone H3 (Lys9) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 86990-2-RR

    ChIP experiment of HeLa using 86990-2-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di-Methyl-Histone H3 (Lys9) (86990-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 86990-2-RR

    WB analysis using 86990-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86990-2-RR (Di-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86990-2-RR

    IF Staining of HeLa using 86990-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H3 (Lys9) antibody (86990-2-RR, Clone: 252046G7 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Di-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody

Di-Methyl-Histone H3 (Lys9) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
Cat No. 86990-2-RR

产品说明书

CloneNo. 252046G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

别名

Di Methyl Histone H3, Di-Methyl Histone H3 (Lys9), Di-Methyl Histone H3 Lys9, Di-Methyl Histone-H3 Lys9, Di-Methyl-Histone H3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected in/ /
Positive ChIP-qPCR detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1250-1:5000
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86990-2-RR targets Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Di Methyl Histone H3, Di-Methyl Histone H3 (Lys9), Di-Methyl Histone H3 Lys9, Di-Methyl Histone-H3 Lys9, Di-Methyl-Histone H3
观测分子量15-17 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

H3K9me2 is a typical transcriptional repressive modification, primarily enriched in heterochromatin regions (such as centromeres, telomeres, and transposon elements), but can also be scattered throughout euchromatin. By recruiting "reader" proteins such as HP1 (Heterochromatin Protein 1), H3K9me2 can establish and stabilize a chromatin condensation state, thereby silencing gene expression.

实验方案

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Lys9) antibody 86990-2-RRDownload protocol
WB protocol for Di-Methyl-Histone H3 (Lys9) antibody 86990-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...