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验证数据展示

  • Dot Blot experiment using 82828-12-RR (same clone as 82828-12-PBS)

    Dot Blot experiment using 82828-12-RR (same clone as 82828-12-PBS)

    Dot blot analysis was used to confirm the specificity of 82828-12-RR (Di-Methyl-Histone H3 (Lys56) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

  • WB analysis using 82828-12-RR (same clone as 82828-12-PBS)

    WB analysis using 82828-12-RR (same clone as 82828-12-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82828-12-RR (HIST1H3A(H3K56me2) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 82828-12-RR (same clone as 82828-12-PBS)

    IF Staining of NIH/3T3 using 82828-12-RR (same clone as 82828-12-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82828-12-RR (same clone as 82828-12-PBS)

    IF Staining of HepG2 using 82828-12-RR (same clone as 82828-12-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody, PBS Only

Di-Methyl-Histone H3 (Lys56) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA
Cat No. 82828-12-PBS

产品说明书

CloneNo. 243027A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Dot Blot, Indirect ELISA

别名

Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

82828-12-PBS targets Di-Methyl-Histone H3 (Lys56) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Dot Blot, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3
观测分子量15-18 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).

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