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验证数据展示

  • Dot Blot experiment of peptide using 84702-1-RR

    Dot Blot experiment of peptide using 84702-1-RR

    Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 84702-1-RR

    ChIP experiment of HeLa using 84702-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di-Methyl-Histone H3 (Arg2) (84702-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • IHC staining of human placenta using 84702-1-RR

    IHC staining of human placenta using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of rat liver using 84702-1-RR

    IHC staining of rat liver using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IHC staining of mouse liver using 84702-1-RR

    IHC staining of mouse liver using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IF Staining of HeLa using 84702-1-RR

    IF Staining of HeLa using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of NIH/3T3 using 84702-1-RR

    IF Staining of NIH/3T3 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of C6 using 84702-1-RR

    IF Staining of C6 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84702-1-RR

    FC experiment of HeLa using 84702-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Di-Methyl-Histone H3 (Arg2) Recombinant monoclonal antibody

Di-Methyl-Histone H3 (Arg2) Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR
Cat No. 84702-1-RR

产品说明书

CloneNo. 241896D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

别名

Di Methyl Histone H3, Di-Methyl Histone H3 (Arg2), Di-Methyl Histone H3 Arg2, Di-Methyl Histone-H3 Arg2, Di-Methyl-Histone H3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat liver tissue, human placenta tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inpeptide
Positive ChIP-qPCR detected inHeLa cells

This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84702-1-RR targets Di-Methyl-Histone H3 (Arg2) in IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 Di Methyl Histone H3, Di-Methyl Histone H3 (Arg2), Di-Methyl Histone H3 Arg2, Di-Methyl Histone-H3 Arg2, Di-Methyl-Histone H3
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672118
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RRDownload protocol
IHC protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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