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DYNC2H1 Polyclonal antibody

DYNC2H1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29758-1-AP

Print datasheet

Synonyms

DHC1b, DHC2, DNCH2, DYH1B, DYNC2H1, FLJ11756, hdhc11

验证数据展示

  • WB analysis of mouse testis using 29758-1-AP

    WB analysis of mouse testis using 29758-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 29758-1-AP (DYNC2H1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 29758-1-AP

    IF Staining of hTERT-RPE1 using 29758-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IF Staining of hTERT-RPE1 using 29758-1-AP

    IF Staining of hTERT-RPE1 using 29758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IHC staining of mouse kidney using 29758-1-AP

    IHC staining of mouse kidney using 29758-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 29758-1-AP

    IHC staining of mouse kidney using 29758-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29758-1-AP targets DYNC2H1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen DYNC2H1 fusion protein Ag30707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynein, cytoplasmic 2, heavy chain 1
Synonyms DHC1b, DHC2, DNCH2, DYH1B, DYNC2H1, FLJ11756, hdhc11
Calculated Molecular Weight 493 kDa
Observed Molecular Weight493 kDa
GenBank Accession NumberNM_001377
Gene Symbol DYNC2H1
Gene ID (NCBI) 79659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCM8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYNC2H1 (Cytoplasmic dynein 2 heavy chain 1) is involved in ciliary intraflagellar transport (IFT). DYNC2H1 drives retrograde transport of the IFT-A protein complex that regulates tip-to-base transport in cilia, involved in the generation and maintenance of cilia. DYNC2H1 variants or retina-predominant variants cause nonsyndromic retinal degeneration (PMID:32753734). DYNC2H1 mutations also cause asphyxiating thoracic dystrophy and short rib-polydactyly syndrome (PMID: 19442771). DYNC2H1 was localized to the basal bodies by immunofluorescence staining (PMID: 21552265, PMID: 26077881, PMID: 30320547).

实验方案

Product Specific Protocols
WB protocol for DYNC2H1 antibody 29758-1-APDownload protocol
IHC protocol for DYNC2H1 antibody 29758-1-APDownload protocol
IF protocol for DYNC2H1 antibody 29758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols