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验证数据展示

  • WB analysis using 12345-1-AP (same clone as 12345-1-PBS)

    WB analysis using 12345-1-AP (same clone as 12345-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12345-1-AP (same clone as 12345-1-PBS)

    IF Staining of MCF-7 using 12345-1-AP (same clone as 12345-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DYNC1H1 antibody (12345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

    IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DYNC1H1 antibody included
  • IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

    IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DYNC1H1 antibody included
  • IP experiment of HeLa using 12345-1-AP (same clone as 12345-1-PBS)

    IP experiment of HeLa using 12345-1-AP (same clone as 12345-1-PBS)

    IP result of anti-DYNC1H1 (IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:5000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

    IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DYNC1H1 antibody included
  • IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

    IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DYNC1H1 antibody included
  • Featured Product
  • KD/KO Validated

DYNC1H1 Polyclonal antibody, PBS Only

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12345-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DYNC1H 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DYNC1H1 Ready-To-Use IHC Kit. DYNC1H1 primary antibody included.

产品信息

12345-1-PBS targets DYNC1H1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2999

Product name: Recombinant human KIAA0325 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 590-989 aa of BC021297

Sequence: MAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, cytoplasmic 1, heavy chain 1
别名 DYNC1H 1
计算分子量 4646 aa, 532 kDa
观测分子量 532 kDa
GenBank蛋白编号BC021297
基因名称 DYNC1H1
Gene ID (NCBI) 1778
RRIDAB_2261765
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14204
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.

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