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验证数据展示

  • ELISA experiment of FusionProtein using Biotin-66008-4

    ELISA experiment of FusionProtein using Biotin-66008-4

    Ag2329 (DYKDDDDK tagged protein) was coated onto microtiter plates at 0.073 µg/well and then incubated with a dilution series of Biotin-66008-4 (start concentration 500 ng/ml). And then detected with Streptavidin Poly-HRP (1:2000) followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm. Negative control is 66008-4-Ig.

  • IF Staining of Transfected HEK-293 using Biotin-66008-4

    IF Staining of Transfected HEK-293 using Biotin-66008-4

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using Biotin DYKDDDDK tag antibody (Biotin-66008-4, Clone: 8H6A10 ) at dilution of 1:600 and Alexa Fluor 488 Streptavidin.

Biotin-conjugated DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for IF/ICC, ELISA
Cat No. Biotin-66008-4

产品说明书

CloneNo. 8H6A10

宿主/亚型

Mouse / IgG2b

种属反应性

recombinant protein

应用

IF/ICC, ELISA

别名

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
Positive ELISA detected inFusionProtein

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:2000-1:4000000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66008-4 targets DYKDDDDK tag in IF/ICC, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Flag Tag
别名 Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK
基因名称 Flag Tag
Gene ID (NCBI) 8
RRIDAB_3086560
偶联类型 Biotin
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
IF protocol for Biotin DYKDDDDK tag antibody Biotin-66008-4Download protocol
Standard Protocols
Click here to view our Standard Protocols