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验证数据展示

  • IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 4ug; Detection:80010-1-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug. This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    FC experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (80010-1-RR, Clone:4K14) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80010-1-RR

    Affinity and Kinetic Characterization of 80010-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80010-1-RR against DYKDDDDK tag were performed. The affinity constant is 83.7 pM.

DYKDDDDK tag Recombinant antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Uni-rAbTM Recombinant Antibody for IF/ICC, IP, Indirect ELISA
Cat No. 80010-1-PBS

产品说明书

CloneNo. 4K14

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

IF/ICC, IP, Indirect ELISA

别名

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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The antibody 80010-1-RR is suggested for IP and IF.

产品信息

80010-1-PBS targets DYKDDDDK tag in IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 recombinant protein
免疫原 DYKDDDDK tag fusion protein Ag2329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Flag Tag
别名 Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK
基因名称 Flag Tag
Gene ID (NCBI) 8
RRIDAB_2882940
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.