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DUS4L Polyclonal antibody

DUS4L Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21870-1-AP

Print datasheet

Synonyms

DUS4, DUS4L, PP35

验证数据展示

  • WB analysis of mouse kidney using 21870-1-AP

    WB analysis of mouse kidney using 21870-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21870-1-AP (DUS4L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 21870-1-AP

    IHC staining of human liver cancer using 21870-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21870-1-AP (DUS4L Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 21870-1-AP

    IHC staining of human liver cancer using 21870-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21870-1-AP (DUS4L Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21870-1-AP is DUS4L Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen DUS4L fusion protein Ag16145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydrouridine synthase 4-like (S. cerevisiae)
Synonyms DUS4, DUS4L, PP35
Calculated Molecular Weight 317 aa, 36 kDa
Observed Molecular Weight 22 kDa, 30 kDa
GenBank Accession NumberBC111774
Gene Symbol DUS4L
Gene ID (NCBI) 11062
RRIDAB_2878933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95620
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

tRNA-dihydrouridine synthase 4-like belongs to the dus family.It catalyzes the synthesis of dihydrouridine and a modified base found in the D-loop of most tRNAs.Researches showed that DUS4L was alsophosphorylated in vitro by a Ca2+ calmodulin-activated kinase. It has 2 isoforms produced by alternative splicing .One, at 35 kDa, comigrated with a second cAMP-stimulated RIS-ROS phosphoprotein,DUS4L,which was also detected in the cytoskeleton.This antibody recognize the C-terminal of DUS4L.

实验方案

Product Specific Protocols
WB protocol for DUS4L antibody 21870-1-APDownload protocol
IHC protocol for DUS4L antibody 21870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols