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验证数据展示

  • WB analysis using 83386-5-RR (same clone as 83386-5-PBS)

    WB analysis using 83386-5-RR (same clone as 83386-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83386-5-RR (DSC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83386-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 83386-5-RR (same clone as 83386-5-PBS)

    IHC staining of mouse skin using 83386-5-RR (same clone as 83386-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83386-5-RR (DSC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83386-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83386-5-RR (same clone as 83386-5-PBS)

    IF Staining of A431 using 83386-5-RR (same clone as 83386-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DSC3 antibody (83386-5-RR, Clone: 240292B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83386-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83386-5-RR

    Affinity and Kinetic Characterization of 83386-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83386-5-RR against Human DSC3 were performed. The affinity constant is below 1 pM.

DSC3 Recombinant monoclonal antibody, PBS Only

DSC3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83386-5-PBS

产品说明书

CloneNo. 240292B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DSC1, DSC, Desmocollin 4, desmocollin 3, CDHF3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83386-5-PBS targets DSC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34648

Product name: Recombinant human DSC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 500-690 aa of NM_001941

Sequence: NRNGNGLRYKKLHDPKGWITIDEISGSIITSKILDREVETPKNELYNITVLAIDKDDRSCTGTLAVNIEDVNDNPPEILQEYVVICKPKMGYTDILAVDPDEPVHGAPFYFSLPNTSPEISRLWSLTKVNDTAARLSYQKNAGFQEYTIPITVKDRAGQAATKLLRVNLCECTHPTQCRATSRSTGVILGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 desmocollin 3
别名 DSC1, DSC, Desmocollin 4, desmocollin 3, CDHF3
计算分子量100 kDa
GenBank蛋白编号NM_001941
基因名称 DSC3
Gene ID (NCBI) 1825
RRIDAB_3671042
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ14574
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Desmocollin-3 (DSC3), also known as CDHF3 and DSC4, is a member of the cadherin superfamily of calcium-dependent cell adhesion molecules and a major component of desmosomes. It has 2 isoforms with molecular masses of 100 and 93 kDa. It is particularly expressed in the vagina, testis, skin, oral mucosa and esophagus. Its subcellular localization is at the cell membrane. Post-translational modifications, including glycosylation, have been described.

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