CRMP4 Polyclonal antibody
CRMP4 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells, Jurkat cells, mouse cerebellum tissue, mouse thymus tissue |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IP | See 1 publications below |
产品信息
18969-1-AP targets CRMP4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat |
Immunogen | CRMP4 fusion protein Ag5299 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | dihydropyrimidinase-like 3 |
Synonyms | CRMP 4, CRMP4, dihydropyrimidinase like 3, DPYSL3, DRP 3, DRP3, LCRMP, ULIP, ULIP 1, ULIP1, Unc 33 like phosphoprotein 1 |
Calculated Molecular Weight | 570 aa, 62 kDa |
Observed Molecular Weight | 60-70 kDa |
GenBank Accession Number | BC039006 |
Gene Symbol | DPYSL3 |
Gene ID (NCBI) | 1809 |
RRID | AB_10603361 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14195 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CRMP4 antibody 18969-1-AP | Download protocol |
IHC protocol for CRMP4 antibody 18969-1-AP | Download protocol |
IF protocol for CRMP4 antibody 18969-1-AP | Download protocol |
IP protocol for CRMP4 antibody 18969-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Free Radic Biol Med Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins. | ||
Biochem Biophys Res Commun Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina. |