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验证数据展示

  • WB analysis of SH-SY5Y using 18969-1-AP

    WB analysis of SH-SY5Y using 18969-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 18969-1-AP

    IP experiment of SH-SY5Y using 18969-1-AP

    IP result of anti-CRMP4 (IP:18969-1-AP, 3ug; Detection:18969-1-AP 1:700) with SH-SY5Y cells lysate 3000ug.

  • IHC staining of human liver cancer using 18969-1-AP

    IHC staining of human liver cancer using 18969-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18969-1-AP (CRMP4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 18969-1-AP

    IHC staining of human liver cancer using 18969-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18969-1-AP (CRMP4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 18969-1-AP

    IHC staining of human colon using 18969-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18969-1-AP (CRMP4 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 18969-1-AP

    IF Staining of HepG2 using 18969-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CRMP4 antibody (18969-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CRMP4 Polyclonal antibody

CRMP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18969-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

DPYSL3, DRP-3, DPYSL 3, Dihydropyrimidinase-related protein 3, CRMP-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, Jurkat cells, mouse cerebellum tissue, mouse thymus tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18969-1-AP targets CRMP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原 CRMP4 fusion protein Ag5299 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dihydropyrimidinase-like 3
别名 DPYSL3, DRP-3, DPYSL 3, Dihydropyrimidinase-related protein 3, CRMP-4
计算分子量 570 aa, 62 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC039006
基因名称 CRMP4
Gene ID (NCBI) 1809
RRIDAB_10603361
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14195
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPYSL3(Dihydropyrimidinase-related protein 3) is also named as CRMP4, DRP3, ULIP, ULIP1 and belongs to the hydantoinase/dihydropyrimidinase subfamily.DPYSL3 is a developmentally regulated protein, strongly expressed in early embryonic post-mitotic neural cells, reaching a peak of expression around the first post-natal week. In the adult brain, DPYSL3 is present mainly in regions that retain neurogenesis.DPYSL3 protein is also found in synaptic sites at the adult neuromuscular junction where it may be involved in the maintenance of neuronal stability(PMID: 16135096).It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CRMP4 antibody 18969-1-APDownload protocol
IHC protocol for CRMP4 antibody 18969-1-APDownload protocol
IF protocol for CRMP4 antibody 18969-1-APDownload protocol
IP protocol for CRMP4 antibody 18969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.

Authors - Jeremy A Whitson
View Article
ratWB, IP

Biochem Biophys Res Commun

Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina.

Authors - Kazuhiro Tokuda
View Article