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CRMP2 Polyclonal antibody

CRMP2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14686-1-AP

Print datasheet

Synonyms

CRMP 2, CRMP2, DHPRP2, dihydropyrimidinase like 2, DPYSL2, DRP 2, DRP2, N2A3, ULIP 2, ULIP2, Unc 33 like phosphoprotein 2

验证数据展示

  • WB analysis using 14686-1-AP

    WB analysis using 14686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14686-1-AP

    IHC staining of mouse brain using 14686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DPYSL2 antibody included
  • IHC staining of mouse brain using 14686-1-AP

    IHC staining of mouse brain using 14686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DPYSL2 antibody included
  • IF Staining of SH-SY5Y using 14686-1-AP

    IF Staining of SH-SY5Y using 14686-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 14686-1-AP (CRMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-12 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DPYSL2 Ready-To-Use IHC Kit. DPYSL2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

14686-1-AP targets CRMP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CRMP2 fusion protein Ag5985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydropyrimidinase-like 2
Synonyms CRMP 2, CRMP2, DHPRP2, dihydropyrimidinase like 2, DPYSL2, DRP 2, DRP2, N2A3, ULIP 2, ULIP2, Unc 33 like phosphoprotein 2
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62-66 kDa
GenBank Accession NumberBC056408
Gene Symbol DPYSL2
Gene ID (NCBI) 1808
RRIDAB_10638925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).

实验方案

Product Specific Protocols
WB protocol for CRMP2 antibody 14686-1-APDownload protocol
IHC protocol for CRMP2 antibody 14686-1-APDownload protocol
IF protocol for CRMP2 antibody 14686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie
View Article
mouseIF

Nat Commun

A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia

Authors - Anna Laddach
View Article