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验证数据展示

  • WB analysis of HepG2 using 11568-1-AP

    WB analysis of HepG2 using 11568-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 11568-1-AP

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 11568-1-AP

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11568-1-AP

    IF Staining of HepG2 using 11568-1-AP

    Immunofluorescent analysis of HepG2 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DPP9 Polyclonal antibody

DPP9 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 11568-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF, IHC, ELISA

别名

dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DP9, DPLP9, DPP IX, DPP9, DPRP 2, DPRP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A2780 cells, MCF-7 cells, mouse heart tissue, PC-3 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11568-1-AP targets DPP9 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DPP9 fusion protein Ag2145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dipeptidyl-peptidase 9
别名 dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DP9, DPLP9, DPP IX, DPP9, DPRP 2, DPRP2
计算分子量 863 aa, 98 kDa
观测分子量 98 kDa
GenBank蛋白编号BC037948
基因名称 DPP9
Gene ID (NCBI) 91039
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86TI2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dipeptidyl peptidase 9(DPP9), is a member of the S9B family in clan SC of the serine proteases. DPP9 has 3 isoforms with MW of 95, 98 and 101 kDa. DPP9 is involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer.