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验证数据展示

  • WB analysis using 12752-1-AP

    WB analysis using 12752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12752-1-AP (DPP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12752-1-AP

    IP experiment of mouse testis using 12752-1-AP

    IP result of anti-DPP8 (IP:12752-1-AP, 4ug; Detection:12752-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

  • IHC staining of human prostate cancer using 12752-1-AP

    IHC staining of human prostate cancer using 12752-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12752-1-AP (DPP8 antibody) at dilution of 1:100 (under 10x lens).

DPP8 Polyclonal antibody

DPP8 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12752-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

dipeptidyl peptidase 8, Dipeptidyl peptidase VIII, DP8, DPP VIII, DPP8, DPRP 1, DPRP1, MSTP135, MSTP141, Prolyl dipeptidase DPP8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12752-1-AP targets DPP8 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3262

Product name: Recombinant human DPP8 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-329 aa of BC030688

Sequence: MWKRSEQMKIKSGKCNMAAAMETEQLGVEIFETADCEENIESQDRPKLEPFYVERYSWSQLKKLLADTRKYHGYMMAKAPHDFMFVKRNDPDGPHSDRIYYLAMSGENRENTLFYSEIPKTINRAAVLMLSWKPLLDLFQATLDYGMYSREEELLRERKRIGTVGIASYDYHQGSGTFLFQAGSGIYHVKDGGPQGFTQQPLRPNLVETSCPNIRMDPKLCPADPDWIAFIHSNDIWISNIVTREERRLTYVHNELANMEEDARSAGVATFVLQEEFDRYSGYWWCPKAETTPSGGKILRILYEENDESEVEIIHVTSPMLETRRADSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dipeptidyl-peptidase 8
别名 dipeptidyl peptidase 8, Dipeptidyl peptidase VIII, DP8, DPP VIII, DPP8, DPRP 1, DPRP1, MSTP135, MSTP141, Prolyl dipeptidase DPP8
计算分子量 847 aa, 97 kDa
观测分子量 75-83 kDa
GenBank蛋白编号BC030688
基因名称 DPP8
Gene ID (NCBI) 54878
RRIDAB_10638438
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6V1X1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPP8 antibody 12752-1-APDownload protocol
IHC protocol for DPP8 antibody 12752-1-APDownload protocol
IP protocol for DPP8 antibody 12752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols