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验证数据展示

  • WB analysis using 68383-1-Ig (same clone as 68383-1-PBS)

    WB analysis using 68383-1-Ig (same clone as 68383-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50051-1

    Sandwich ELISA standard curve of MP50051-1

    Sandwich ELISA standard curve of MP50051-1, DPP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68383-1-PBS. Detection antibody: 68383-2-PBS. Standard: Eg0157. Range: 0.313-20 ng/mL.

  • Sample test of MP50051-1

    Sample test of MP50051-1

    Urine of eight individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 33.23 ng/mL with a range of 9.07-105.84 ng/mL.

  • Sample test of MP50051-1

    Sample test of MP50051-1

    Saliva of eight individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 33.57 ng/mL with a range of 21.27-77.69 ng/mL.

  • Sample test of MP50051-1

    Sample test of MP50051-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean DPP4/CD26 concentration was determined to be 0.17 ng/mL in unstimulated PBMC supernatant, 1.84 ng/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP50051-1

    Sample test of MP50051-1

    Serum of sixteen individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 475.10 ng/mL with a range of 189.59-990.11 ng/mL.

  • IF Staining of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

    IF Staining of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using DPP4/CD26 antibody (68383-1-Ig, Clone: 2G6D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

DPP4/CD26 Monoclonal antibody, PBS Only (Capture)

DPP4/CD26 Monoclonal Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68383-1-PBS

产品说明书

CloneNo. 2G6D11

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, rabbit

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD26, 2G6D11, ADABP, ADCP 2, ADCP2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68383-1-PBS targets DPP4/CD26 as part of a matched antibody pair:

MP50051-1: 68383-1-PBS capture and 68383-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, pig, rabbit
免疫原

CatNo: Eg0157

Product name: Recombinant Human DPP4/CD26 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 34-766 aa of BC013329

Sequence: DATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dipeptidyl-peptidase 4
别名 CD26, 2G6D11, ADABP, ADCP 2, ADCP2
计算分子量 88 kDa
观测分子量110 kDa
GenBank蛋白编号BC013329
基因名称 DPP4
Gene ID (NCBI) 1803
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP27487
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

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