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DPM1 Polyclonal antibody

DPM1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12403-2-AP

Print datasheet

Synonyms

CDGIE, DPM synthase, DPM1, Mannose P dolichol synthase, MPD synthase, MPDS

验证数据展示

  • WB analysis of HeLa using 12403-2-AP

    WB analysis of HeLa using 12403-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12403-2-AP (DPM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 12403-2-AP

    IHC staining of human liver cancer using 12403-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12403-2-AP (DPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12403-2-AP targets DPM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen DPM1 fusion protein Ag1128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
Synonyms CDGIE, DPM synthase, DPM1, Mannose P dolichol synthase, MPD synthase, MPDS
Calculated Molecular Weight 260 aa, 30 kDa
Observed Molecular Weight 27-32 kDa
GenBank Accession NumberBC007073
Gene Symbol DPM1
Gene ID (NCBI) 8813
RRIDAB_2093270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPM1 antibody 12403-2-APDownload protocol
IHC protocol for DPM1 antibody 12403-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Genet

Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm.

Authors - Zhiyang Zhou
View Article
WB

FEBS Lett

Aspirin damages the cell wall of Saccharomyces cerevisiae by inhibiting the expression and activity of dolichol-phosphate mannose synthase 1.

Authors - Ming Li
View Article