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验证数据展示

  • WB analysis using 18987-1-AP

    WB analysis using 18987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18987-1-AP

    IHC staining of human kidney using 18987-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 18987-1-AP

    IHC staining of human kidney using 18987-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of mouse brain using 18987-1-AP

    IP experiment of mouse brain using 18987-1-AP

    IP result of anti-DOCK9 (IP:18987-1-AP, 4ug; Detection:18987-1-AP 1:800) with mouse brain tissue lysate 4000ug.

  • IF Staining of HeLa using 18987-1-AP

    IF Staining of HeLa using 18987-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK9 antibody (18987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DOCK9 Polyclonal antibody

DOCK9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18987-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Zizimin-1, ZIZ1, dedicator of cytokinesis 9, Cdc42 guanine nucleotide exchange factor zizimin-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, human brain tissue, mouse brain tissue, human heart tissue, human placenta tissue, HeLa cells, MCF-7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18987-1-AP targets DOCK9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 9
别名 Zizimin-1, ZIZ1, dedicator of cytokinesis 9, Cdc42 guanine nucleotide exchange factor zizimin-1
计算分子量 239 kDa
观测分子量 200-236 kDa
GenBank蛋白编号NM_015296
基因名称 DOCK9
Gene ID (NCBI) 23348
RRIDAB_10596777
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZ29
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

实验方案

Product Specific Protocols
WB protocol for DOCK9 antibody 18987-1-APDownload protocol
IHC protocol for DOCK9 antibody 18987-1-APDownload protocol
IF protocol for DOCK9 antibody 18987-1-APDownload protocol
IP protocol for DOCK9 antibody 18987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Commun Biol

Brain endothelial cells promote breast cancer cell extravasation to the brain via EGFR-DOCK4-RAC1 signalling

Authors - Chiara Galloni
View Article