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验证数据展示

  • FC experiment of HeLa using CL488-67842

    FC experiment of HeLa using CL488-67842

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DOCK7 (CL488-67842, Clone:1A4A6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DOCK7 Monoclonal antibody

DOCK7 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67842

产品说明书

CloneNo. 1A4A6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

dedicator of cytokinesis 7, DOCK7, KIAA1771, ZIR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DOCK7 Ready-To-Use IHC Kit. DOCK7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67842-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67842 targets DOCK7 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag28417

Product name: Recombinant human DOCK7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 447-798 aa of BC016392

Sequence: MAGMYEAVNEVYKVLIPIHEANRDAKKLSTIHGKLQEAFSKIVHQDGKRMFGTYFRVGFYGTKFGDLDEQEFVYKEPAITKLAEISHRLEGFYGERFGEDVVEVIKDSNPVDKCKLDPNKAYIQITYVEPYFDTYEMKDRITYFDKNYNLRRFMYCTPFTLDGRAHGELHEQFKRKTILTTSHAFPYIKTRVNVTHKEEIILTPIEVAIEDMQKKTQELAFATHQDPADPKMLQMVLQGSVGTTVNQGPLEVAQVFLSEIPSDPKLFRHHNKLRLCFKDFTKRCEDALRKNKSLIGPDQKEYQRELERNYHRLKEALQPLINRKIPQLYKAVLPVTCHRDSFSRMSLRKMDL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 dedicator of cytokinesis 7
别名 dedicator of cytokinesis 7, DOCK7, KIAA1771, ZIR2
计算分子量 2109 aa, 239 kDa
观测分子量243 kDa
GenBank蛋白编号BC016392
基因名称 DOCK7
Gene ID (NCBI) 85440
RRIDAB_2923798
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96N67
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DOCK180-related protein superfamily. Expressed mainly in neuronal cells, DOCK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DOCK 7 exist due to alternative splicing events.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DOCK7 antibody CL488-67842Download protocol
Standard Protocols
Click here to view our Standard Protocols