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验证数据展示

  • WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

    WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 29938-1-AP (same clone as 29938-1-PBS)

    WB analysis of A549 using 29938-1-AP (same clone as 29938-1-PBS)

    WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

  • IP experiment of DU 145 using 29938-1-AP (same clone as 29938-1-PBS)

    IP experiment of DU 145 using 29938-1-AP (same clone as 29938-1-PBS)

    IP result of anti-DOCK5 (IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 29938-1-AP (same clone as 29938-1-PBS)

    IHC staining of mouse kidney using 29938-1-AP (same clone as 29938-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DOCK5 antibody included
  • IF Staining of U2OS using 29938-1-AP (same clone as 29938-1-PBS)

    IF Staining of U2OS using 29938-1-AP (same clone as 29938-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DOCK5 Polyclonal antibody, PBS Only

DOCK5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 29938-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

dedicator of cytokinesis 5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DOCK5 Ready-To-Use IHC Kit. DOCK5 primary antibody included.

产品信息

29938-1-PBS targets DOCK5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32193

Product name: Recombinant human DOCK5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1700-1870 aa of BC137175

Sequence: ILEPLLERRASSGARVEDLSLREENSENRISKFKRKDWSLSKSQVIAEKAPEPDLMSPTRKAQRPKSLQLMDNRLSPFHGSSPPQSTPLSPPPLTPKATRTLSSPSLQTDGIAATPVPPPPPPKSKPYEGSQRNSTELAPPLPVRREAKAPPPPPPKARKSGIPTSEPGSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 5
别名 dedicator of cytokinesis 5
计算分子量 1870 aa, 215 kDa
观测分子量190 kDa
GenBank蛋白编号BC137175
基因名称 DOCK5
Gene ID (NCBI) 80005
RRIDAB_3086194
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7D0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.

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