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CoraLite® Plus 488-conjugated DOCK4 Polyclonal antibody

DOCK4 Polyclonal Antibody for IF/ICC,FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC,FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-27151

Print datasheet

Synonyms

dedicator of cytokinesis 4, DOCK4, KIAA0716

验证数据展示

  • IF Staining of HeLa using CL488-27151

    IF Staining of HeLa using CL488-27151

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DOCK4 antibody (CL488-27151) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-27151

    FC experiment of HeLa using CL488-27151

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DOCK4 (CL488-27151)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 27151-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27151 targets DOCK4 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC,FC (Intra)
Tested Reactivity human
Immunogen DOCK4 fusion protein Ag25946 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dedicator of cytokinesis 4
Synonyms dedicator of cytokinesis 4, DOCK4, KIAA0716
Calculated Molecular Weight 2011 aa, 230 kDa
Observed Molecular Weight 225 kDa
GenBank Accession NumberBC117689
Gene Symbol DOCK4
Gene ID (NCBI) 9732
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1I0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DOCK4 antibody CL488-27151Download protocol
FC protocol for CL Plus 488 DOCK4 antibody CL488-27151Download protocol
Standard Protocols
Click here to view our Standard Protocols