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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 20245-1-AP

    WB analysis using 20245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20245-1-AP (DOCK2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 20245-1-AP

    IF Staining of Jurkat using 20245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DOCK2-Specific antibody (20245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DOCK2-Specific Polyclonal antibody

DOCK2-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20245-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

dedicator of cytokinesis 2, DOCK2, DOCK2-Specific, KIAA0209

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Ramos cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20245-1-AP targets DOCK2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 2
别名 dedicator of cytokinesis 2, DOCK2, DOCK2-Specific, KIAA0209
计算分子量 212 kDa
观测分子量212 kDa
GenBank蛋白编号NM_004946
基因名称 DOCK2
Gene ID (NCBI) 1794
RRIDAB_10699879
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92608
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.

实验方案

Product Specific Protocols
WB protocol for DOCK2-Specific antibody 20245-1-APDownload protocol
IF protocol for DOCK2-Specific antibody 20245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols