验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, Ramos cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
66969-1-Ig targets DOCK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | DOCK2 fusion protein Ag28582 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dedicator of cytokinesis 2 |
| 别名 | dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209 |
| 计算分子量 | 212 kDa, 38 kDa |
| 观测分子量 | 200-210 kDa |
| GenBank蛋白编号 | BC016996 |
| 基因名称 | DOCK2 |
| Gene ID (NCBI) | 1794 |
| RRID | AB_2882291 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q92608 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DOCK2 antibody 66969-1-Ig | Download protocol |
| IHC protocol for DOCK2 antibody 66969-1-Ig | Download protocol |
| IF protocol for DOCK2 antibody 66969-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
