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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68485-1-Ig (same clone as 68485-1-PBS)

    WB analysis using 68485-1-Ig (same clone as 68485-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68485-1-Ig (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68485-1-PBS in a different storage buffer formulation.

DNMT1 Monoclonal antibody, PBS Only

DNMT1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68485-1-PBS

产品说明书

CloneNo. 2G5C5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, Indirect ELISA

别名

AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, FLJ16293, M.HsaI, MCMT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68485-1-PBS targets DNMT1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag19116

Product name: Recombinant human DNMT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1288-1632 aa of BC126227

Sequence: VSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DNA (cytosine-5-)-methyltransferase 1
别名 AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, FLJ16293, M.HsaI, MCMT
计算分子量 1632 aa, 185 kDa
观测分子量180-200 kDa
GenBank蛋白编号BC126227
基因名称 DNMT1
Gene ID (NCBI) 1786
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP26358
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)