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验证数据展示

  • WB analysis using 85157-4-RR (same clone as 85157-4-PBS)

    WB analysis using 85157-4-RR (same clone as 85157-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85157-4-RR (DNAJC7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85157-4-RR (same clone as 85157-4-PBS)

    IHC staining of mouse brain using 85157-4-RR (same clone as 85157-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85157-4-RR (DNAJC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJC7 antibody included
  • IF Staining of HepG2 using 85157-4-RR (same clone as 85157-4-PBS)

    IF Staining of HepG2 using 85157-4-RR (same clone as 85157-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJC7 antibody (85157-4-RR, Clone: 242875B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85157-4-RR (same clone as 85157-4-PBS)

    FC experiment of HeLa using 85157-4-RR (same clone as 85157-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNAJC7 Recombinant antibody (85157-4-RR, Clone:242875B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85157-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85157-4-RR

    Affinity and Kinetic Characterization of 85157-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85157-4-RR against Human DNAJC7 were performed. The affinity constant is 0.767 nM.

DNAJC7 Recombinant monoclonal antibody, PBS Only

DNAJC7 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85157-4-PBS

产品说明书

CloneNo. 242875B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

242875B3, CCRP, DANJC7, DNAJC 7, TPR2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DNAJC7 Ready-To-Use IHC Kit. DNAJC7 primary antibody included.

产品信息

85157-4-PBS targets DNAJC7 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1572

Product name: Recombinant human DNAJC7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 145-494 aa of BC011837

Sequence: YEKIAETDFEKRDFRKVVFCMDRALEFAPACHRFKILKAECLAMLGRYPEAQSVASDILRMDSTNADALYVRGLCLYYEDCIEKAVQFFVQALRMAPDHEKACIACRNAKALKAKKEDGNKAFKEGNYKLAYELYTEALGIDPNNIKTNAKLYCNRGTVNSKLRKLDDAIEDCTNAVKLDDTYIKAYLRRAQCYMDTEQYEEAVRDYEKVYQTEKTKEHKQLLKNAQLELKKSKRKDYYKILGVDKNASEDEIKKAYRKRALMHHPDRHSGASAEVQKEEEKKFKEVGEAFTILSDPKKKTRYDSGQDLDEEGMNMGDFDPNNIFKAFFGGPGGFSFEASGPGNFFFQFG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 7
别名 242875B3, CCRP, DANJC7, DNAJC 7, TPR2
计算分子量 56 kDa
观测分子量56 kDa
GenBank蛋白编号BC011837
基因名称 DNAJC7
Gene ID (NCBI) 7266
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99615
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.

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