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验证数据展示

  • WB analysis using 32204-1-AP (same clone as 32204-1-PBS)

    WB analysis using 32204-1-AP (same clone as 32204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32204-1-AP (DNAJC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 32204-1-AP (same clone as 32204-1-PBS)

    IHC staining of mouse cerebellum using 32204-1-AP (same clone as 32204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJC5 antibody included
  • IHC staining of mouse pancreas using 32204-1-AP (same clone as 32204-1-PBS)

    IHC staining of mouse pancreas using 32204-1-AP (same clone as 32204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJC5 antibody included
  • IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

    IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJC5 antibody included
  • IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

    IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJC5 antibody included
  • IHC staining of mouse lung using 32204-1-AP (same clone as 32204-1-PBS)

    IHC staining of mouse lung using 32204-1-AP (same clone as 32204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJC5 antibody included
  • IHC staining of rat liver using 32204-1-AP (same clone as 32204-1-PBS)

    IHC staining of rat liver using 32204-1-AP (same clone as 32204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJC5 antibody included
  • IF Staining of HeLa using 32204-1-AP (same clone as 32204-1-PBS)

    IF Staining of HeLa using 32204-1-AP (same clone as 32204-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC5 antibody (32204-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

DNAJC5 Polyclonal antibody, PBS Only

DNAJC5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32204-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Ceroid-lipofuscinosis neuronal protein 4, CLN4, CSP, Cysteine string protein, DnaJ (Hsp40) Homolog, Subfamily C, Member 5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

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Planning an IHC experiment? We recommend our IHCeasy DNAJC5 Ready-To-Use IHC Kit. DNAJC5 primary antibody included.

产品信息

32204-1-PBS targets DNAJC5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35443

Product name: Recombinant human DNAJC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-198 aa of NM_025219.2

Sequence: FVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 5
别名 Ceroid-lipofuscinosis neuronal protein 4, CLN4, CSP, Cysteine string protein, DnaJ (Hsp40) Homolog, Subfamily C, Member 5
计算分子量22kDa，198aa
观测分子量30 kDa
GenBank蛋白编号NM_025219.2
基因名称 DNAJC5
Gene ID (NCBI) 80331
RRIDAB_3670222
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H3Z4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJC5 (DnaJ homolog subfamily C member 5), also known as cysteine string protein α (CSPα), is a member of the HSP40 (DNAJ) family of proteins, as well as a co-chaperone of HSC70, and has been shown to control the extracellular release of many neurodegenerative disease proteins. DNAJC5 contains three structural domains - -a common N-terminal J structural domain conserved in DNAJ proteins, the cysteine string (CS) central structural domain, which is highly palmitoylated and anchors the protein to late endosomes, and an overall disordered C-terminal structural domain. Mutations in DNAJC5 have been strongly implicated in the development of human diseases such as neuronal waxy lipofuscinosis, cystic fibrosis, Huntington's disease and Parkinson's disease.

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