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验证数据展示

  • IHC staining of human breast cancer using 13853-1-AP

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human breast cancer using 13853-1-AP

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of MCF-7 using 13853-1-AP

    WB analysis of MCF-7 using 13853-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

DNAJC4 Polyclonal antibody

DNAJC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13853-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13853-1-AP targets DNAJC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4826

Product name: Recombinant human DNAJC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC044584

Sequence: MPPLLPLRLCRLWPRNPPSRLLGAAAGQRSRPSTYYELLGVHPGASTEEVKRAFFSKSKELHPDRDPGNPSLHSRFVELSEAYRVLSREQSRRSYDDQLRSGSPPKSPRTTVHDKSAHQTHSSWTPPNAQYWSQFHSVRPQGPQLRQQQHKQNKQVLGYCLLLMLAGMGLHYIAFRKVKQMHLNFMDEKDRIITAFYNEARARARANRGILQQERQRLGQRQPPPSEPTQGPEIVPRGAGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 4
别名 DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC044584
基因名称 DNAJC4
Gene ID (NCBI) 3338
RRIDAB_2095224
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NNZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJC4 antibody 13853-1-APDownload protocol
IHC protocol for DNAJC4 antibody 13853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols