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验证数据展示

  • IP experiment of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

    IP experiment of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

    IP result of anti-DNAJB14 (IP:16501-1-AP, 4ug; Detection:16501-1-AP 1:1000) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

  • WB analysis using 16501-1-AP (same clone as 16501-1-PBS)

    WB analysis using 16501-1-AP (same clone as 16501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 16501-1-AP (same clone as 16501-1-PBS)

    IHC staining of human ovary cancer using 16501-1-AP (same clone as 16501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16501-1-AP (DNAJB14 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

    IF Staining of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJB14 antibody (16501-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

DNAJB14 Polyclonal antibody, PBS Only

DNAJB14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16501-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EGNR9427, PRO34683, UNQ9427/PRO34683

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16501-1-PBS targets DNAJB14 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9668

Product name: Recombinant human DNAJB14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-254 aa of BC022248

Sequence: MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNSPHCRKPSGSGDQSKPNCTKDSTSGSGEGGKGYTKDQVDGVLSINKCKNYYEVLGVTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNEEQACNHQNNGRFNFHRGCEADITPEDLFNIFFGGGFPSGSVHSFSNGRAGYSQQHQHRHSGHEREEERGDGGFSVFIQLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 14
别名 EGNR9427, PRO34683, UNQ9427/PRO34683
计算分子量 379 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC022248
基因名称 DNAJB14
Gene ID (NCBI) 79982
RRIDAB_2094414
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBM8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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