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验证数据展示

  • WB analysis using 30073-1-AP

    WB analysis using 30073-1-AP

    mouse testis tissue were subjected to Tris-Acetate gel system followed by western blot with 30073-1-AP (DNAH6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 30073-1-AP

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30073-1-AP

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of hTERT-RPE1 using 30073-1-AP

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IF Staining of hTERT-RPE1 using 30073-1-AP

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

DNAH6 Polyclonal antibody

DNAH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30073-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Ciliary dynein heavy chain 6, Dnahc6, DNHL1, Dynein axonemal heavy chain 6, Dynein heavy chain 6, axonemal

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30073-1-AP targets DNAH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32364

Product name: Recombinant human DNAH6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 148-408 aa of BC104884

Sequence: NNKLEGKTCGTGPSLAAVFEDDKNFHTIISQIKETIQAAFESARIYAATFEKFQIFFKENESLDLQALKLQEPDINFFSEQLEKYHKQHKDAVALRPTRNVGLLLIDTRLLREKLIPSPLRCLEVLNFMLPRQSKKKVDAIIFEAQDAEYKLEFVPTTTTEYVHSLLFLDEIQERIESLEDEGNIVTQMYKLMEQYQVPTPPEDFAVFATMKPSIVAVRNAIDKSVGDRESSIKQFCVHLGSDLEELNNEVNEVKLQAQVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, axonemal, heavy chain 6
别名 Ciliary dynein heavy chain 6, Dnahc6, DNHL1, Dynein axonemal heavy chain 6, Dynein heavy chain 6, axonemal
计算分子量475 kDa
观测分子量 475 kDa
GenBank蛋白编号BC104884
基因名称 DNAH6
Gene ID (NCBI) 1768
RRIDAB_2935510
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0G6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.

实验方案

Product Specific Protocols
IF protocol for DNAH6 antibody 30073-1-APDownload protocol
IHC protocol for DNAH6 antibody 30073-1-APDownload protocol
WB protocol for DNAH6 antibody 30073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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