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验证数据展示

  • WB analysis using 16711-1-AP (same clone as 16711-1-PBS)

    WB analysis using 16711-1-AP (same clone as 16711-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16711-1-AP (DMPK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 16711-1-AP (same clone as 16711-1-PBS)

    IF Staining of U-251 using 16711-1-AP (same clone as 16711-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DMPK antibody (16711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 16711-1-AP (same clone as 16711-1-PBS)

    IHC staining of mouse skeletal muscle using 16711-1-AP (same clone as 16711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 16711-1-AP (same clone as 16711-1-PBS)

    IHC staining of mouse skeletal muscle using 16711-1-AP (same clone as 16711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • IF Staining of HSkMC using 16711-1-AP (same clone as 16711-1-PBS)

    IF Staining of HSkMC using 16711-1-AP (same clone as 16711-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using DMPK antibody (16711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

DMPK Polyclonal antibody, PBS Only

DMPK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16711-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DM, DM kinase, DM1, DM1 protein kinase, DM1PK

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16711-1-PBS targets DMPK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10127

Product name: Recombinant human DMPK protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 306-611 aa of BC062553

Sequence: VPEEARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDFEGATDTCNFDLVEDGLTAMVSGGGETLSDIREGAPLGVHLPFVGYSYSCMALRDSEVPGPTPMELEAEQLLEPHVQAPSLEPSVSPQDETAEVAVPAAVPAAEAEAEVTLRELQEALEEEVLTRQSLSREMEAIRTDNQNFASQLREAEARNRDLEAHVRQLQERMELLQAEGATAVTGVPSPRATDPPSHLDGPPAVAVGQCPLVGPGPMHRRHLLLPARVPRPGLSEALSLLLFAVVLSRAAALGCIGLVA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dystrophia myotonica-protein kinase
别名 DM, DM kinase, DM1, DM1 protein kinase, DM1PK
计算分子量 629 aa, 69 kDa
观测分子量 69-75 kDa
GenBank蛋白编号BC062553
基因名称 DMPK
Gene ID (NCBI) 1760
RRIDAB_3741892
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ09013
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DMPK (Myotonic Dystrophy Protein Kinase) is a serine-threonine protein kinase, strongly linked to myotonic dystrophy type 1 (DM1), the most prevalent muscular dystrophy in adults (PMID: 19309729). It is best known for its association with myotonic dystrophy type 1 (DM1), a multisystemic genetic disorder characterized by myotonia, muscle wasting, cardiac conduction defects, and other systemic manifestations (PMID: 24136222).

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