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验证数据展示

  • WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

    WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

    WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

    WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLD antibody included
  • IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLD antibody included
  • Featured Product
  • KD/KO验证

DLD Monoclonal antibody, PBS Only

DLD Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67702-1-PBS

产品说明书

CloneNo. 2E7G5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2E7G5, DLDH, EC:1.8.1.4, GCSL, LAD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DLD Ready-To-Use IHC Kit. DLD primary antibody included.

产品信息

67702-1-PBS targets DLD in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9680

Product name: Recombinant human DLD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 160-509 aa of BC018696

Sequence: NQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydrolipoamide dehydrogenase
别名 2E7G5, DLDH, EC:1.8.1.4, GCSL, LAD
计算分子量 509 aa, 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC018696
基因名称 DLD
Gene ID (NCBI) 1738
RRIDAB_2882894
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP09622
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.