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验证数据展示

  • WB analysis using 66894-1-Ig (same clone as 66894-1-PBS)

    WB analysis using 66894-1-Ig (same clone as 66894-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66894-1-Ig (DLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • IF Staining of A375 using 66894-1-Ig (same clone as 66894-1-PBS)

    IF Staining of A375 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    IF Staining of HepG2 using 66894-1-Ig (same clone as 66894-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DLC1 antibody (66894-1-Ig, Clone: 1G10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66894-1-PBS in a different storage buffer formulation.

DLC1 Monoclonal antibody, PBS Only

DLC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66894-1-PBS

产品说明书

CloneNo. 1G10H12

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DLC1 Ready-To-Use IHC Kit. DLC1 primary antibody included.

产品信息

66894-1-PBS targets DLC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag24404

Product name: Recombinant human DLC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 111-370 aa of BC054511

Sequence: SDEDNNADLCLTDDKQVLNTQGQKTSGQHMIQGAGSLEKALPIIQSNQVSSNSWGIAGETELALVKESGERKVTDSISKSLELCNEISLSEIKDAPKVNAVDTLNVKDIAPEKQLLNSAVIAQQRRKPDPPKDENERSTCNVVQDEFLDTPCTNRGLPLLKTDFGSCLLQPPSCPNGMSAENGLEKSGFSQHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deleted in liver cancer 1
别名 ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, FLJ21120, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12
计算分子量 171 kDa
观测分子量 115 kDa
GenBank蛋白编号BC054511
基因名称 DLC1
Gene ID (NCBI) 10395
RRIDAB_2882223
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96QB1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Deleted in Liver Cancer 1 (DLC1) is a RhoGAP-containing tumor suppressor that inhibits angiogenesis by repressing VEGF production in epithelial cells. DLC1 is often down-regulated in a variety of cancers, such as those of the liver, lung, breast, stomach, brain, colon and prostate due to either genomic deletion or aberrant DNA methylation.

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