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DIS3 Polyclonal antibody

DIS3 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29664-1-AP

Print datasheet

Synonyms

bA555G22.1, DIS3, dis3p, DKFZp667L1817, EXOSC11, FLJ10484, KIAA1008, Protein DIS3 homolog, RP11 342J4.3, RRP44

验证数据展示

  • WB analysis using 29664-1-AP

    WB analysis using 29664-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 29664-1-AP (DIS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29664-1-AP

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIS3 antibody included
  • IHC staining of mouse testis using 29664-1-AP

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIS3 antibody included
  • IF Staining of HeLa using 29664-1-AP

    IF Staining of HeLa using 29664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (29664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue, human colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DIS3 Ready-To-Use IHC Kit. DIS3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29664-1-AP targets DIS3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen DIS3 fusion protein Ag31711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DIS3 mitotic control homolog (S. cerevisiae)
Synonyms bA555G22.1, DIS3, dis3p, DKFZp667L1817, EXOSC11, FLJ10484, KIAA1008, Protein DIS3 homolog, RP11 342J4.3, RRP44
Calculated Molecular Weight109KD
Observed Molecular Weight110 kDa
GenBank Accession NumberNM_014953
Gene Symbol DIS3
Gene ID (NCBI) 22894
RRIDAB_2918336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2L1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.

实验方案

Product Specific Protocols
WB protocol for DIS3 antibody 29664-1-APDownload protocol
IHC protocol for DIS3 antibody 29664-1-APDownload protocol
IF protocol for DIS3 antibody 29664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols