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验证数据展示

  • IHC staining of human colon cancer using 29664-1-AP

    IHC staining of human colon cancer using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIS3 antibody included
  • WB analysis using 29664-1-AP

    WB analysis using 29664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29664-1-AP (DIS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29664-1-AP

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIS3 antibody included
  • IHC staining of mouse testis using 29664-1-AP

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIS3 antibody included
  • IHC staining of mouse testis using 29664-1-AP

    IHC staining of mouse testis using 29664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DIS3 antibody included
  • IF Staining of HeLa using 29664-1-AP

    IF Staining of HeLa using 29664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (29664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

DIS3 Polyclonal antibody

DIS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29664-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DIS 3, Dis-3, dis3p, EC:3.1.13.-, EXOSC11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DIS3 Ready-To-Use IHC Kit. DIS3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29664-1-AP targets DIS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag31711

Product name: Recombinant human DIS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 607-958 aa of NM_014953

Sequence: TTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DIS3 mitotic control homolog (S. cerevisiae)
别名 DIS 3, Dis-3, dis3p, EC:3.1.13.-, EXOSC11
计算分子量109KD
观测分子量110 kDa
GenBank蛋白编号NM_014953
基因名称 DIS3
Gene ID (NCBI) 22894
RRIDAB_2918336
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2L1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.

实验方案

Product Specific Protocols
IF protocol for DIS3 antibody 29664-1-APDownload protocol
IHC protocol for DIS3 antibody 29664-1-APDownload protocol
WB protocol for DIS3 antibody 29664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

PABPC1 mediates degradation of C9orf72-FTLD/ALS GGGGCC repeat RNA

Authors - Ryota Uozumi
View Article
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